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tre3g egfp vector  (TaKaRa)


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    TaKaRa tre3g egfp vector
    Tre3g Egfp Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tre3g+egfp+vector/us11414677-652-0-14
    Average 86 stars, based on 1 article reviews
    tre3g egfp vector - by Bioz Stars, 2026-09
    86/100 stars

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    Amplification:

    Article Title: Methods and compositions for performing continuous directed evolution
    Article Snippet: A fragment containing TPL.AdProt was obtained from pLVX.Tight.TPL.AdProt.Puro by digestion with EcoRI and BamHI and ligated into the pLVX.TRE3G vector (Takara Bio) to create the pLVX.TRE3G.AdProt vector. .. Tre3G.Egfp Vector: A 762 bp fragment containing eGFP was amplified from the eGFP-N3 vector (Takara Bio) using the primers NotI.eGFP.Forward and EcoRI.eGFP.Reverse (TABLE 5) and ligated into the pLVX-TRE3G vector (Takara Bio) using NotI and EcoRI to create the pLVX-TRE3G.eGFP vector. tTA Variant Vectors: A 743 bp fragment containing mCherry was amplified from a pcDNA3.1-mCherry template plasmid using the primers NotI.mCherry.Forward and XhoI.mCherry.Reverse (TABLE 5) and inserted into the pBudCE4.1 vector (Thermo Fisher) using NotI and XhoI to create the pBud.mCherry vector. .. A 771 bp fragment containing tTA was amplified from a tTA.mCherry adenoviral vector using the primers SalI.TTA.Forward and BamHI.TTA.Reverse (TABLE 5) and inserted into the pBud.mCherry vector using BamHI and SalI to create the pBud.tTA.mCherry vector.

    Variant Assay:

    Article Title: Methods and compositions for performing continuous directed evolution
    Article Snippet: A fragment containing TPL.AdProt was obtained from pLVX.Tight.TPL.AdProt.Puro by digestion with EcoRI and BamHI and ligated into the pLVX.TRE3G vector (Takara Bio) to create the pLVX.TRE3G.AdProt vector. .. Tre3G.Egfp Vector: A 762 bp fragment containing eGFP was amplified from the eGFP-N3 vector (Takara Bio) using the primers NotI.eGFP.Forward and EcoRI.eGFP.Reverse (TABLE 5) and ligated into the pLVX-TRE3G vector (Takara Bio) using NotI and EcoRI to create the pLVX-TRE3G.eGFP vector. tTA Variant Vectors: A 743 bp fragment containing mCherry was amplified from a pcDNA3.1-mCherry template plasmid using the primers NotI.mCherry.Forward and XhoI.mCherry.Reverse (TABLE 5) and inserted into the pBudCE4.1 vector (Thermo Fisher) using NotI and XhoI to create the pBud.mCherry vector. .. A 771 bp fragment containing tTA was amplified from a tTA.mCherry adenoviral vector using the primers SalI.TTA.Forward and BamHI.TTA.Reverse (TABLE 5) and inserted into the pBud.mCherry vector using BamHI and SalI to create the pBud.tTA.mCherry vector.

    Plasmid Preparation:

    Article Title: Methods and compositions for performing continuous directed evolution
    Article Snippet: A fragment containing TPL.AdProt was obtained from pLVX.Tight.TPL.AdProt.Puro by digestion with EcoRI and BamHI and ligated into the pLVX.TRE3G vector (Takara Bio) to create the pLVX.TRE3G.AdProt vector. .. Tre3G.Egfp Vector: A 762 bp fragment containing eGFP was amplified from the eGFP-N3 vector (Takara Bio) using the primers NotI.eGFP.Forward and EcoRI.eGFP.Reverse (TABLE 5) and ligated into the pLVX-TRE3G vector (Takara Bio) using NotI and EcoRI to create the pLVX-TRE3G.eGFP vector. tTA Variant Vectors: A 743 bp fragment containing mCherry was amplified from a pcDNA3.1-mCherry template plasmid using the primers NotI.mCherry.Forward and XhoI.mCherry.Reverse (TABLE 5) and inserted into the pBudCE4.1 vector (Thermo Fisher) using NotI and XhoI to create the pBud.mCherry vector. .. A 771 bp fragment containing tTA was amplified from a tTA.mCherry adenoviral vector using the primers SalI.TTA.Forward and BamHI.TTA.Reverse (TABLE 5) and inserted into the pBud.mCherry vector using BamHI and SalI to create the pBud.tTA.mCherry vector.



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